MEK4 Antibody (YA4967)

MEK4 Antibody (YA4967) is a mouse-derived and non-conjugated monoclonal antibody, targeting to MEK-4. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.

MEK4 is a dual specificity protein kinase that functions as an essential component of the MAP kinase signal transduction pathway. It plays a critical role in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. Together with MAP2K7/MKK7, MEK4 is one of the only known kinases that directly activates the stress-activated protein kinases/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2, and MAPK10/JNK3. Both MAP2K4/MKK4 and MAP2K7/MKK7 activate JNKs through phosphorylation but differ in their preference for phosphorylation sites within the Thr-Pro-Tyr motif: MAP2K4 preferentially phosphorylates the Tyr residue, while MAP2K7/MKK7 targets the Thr residue. Phosphorylation of the Thr residue by MAP2K7/MKK7 appears to be a prerequisite for JNK activation, at least in response to pro-inflammatory cytokines, whereas other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 to synergistically phosphorylate JNKs. MEK4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in the mitochondrial death signaling pathway, including cytochrome c release, leading to apoptosis. Unlike MAP2K7/MKK7, which exclusively activates JNKs, MAP2K4/MKK4 additionally activates p38 MAPKs (MAPK11, MAPK12, MAPK13, and MAPK14).

P45985

Predicted band size: 44 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human MEK-4 expressed in E. Coli.