2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. MDM2 Antibody (YA3442)

MDM2 Antibody (YA3442) is a mouse-derived non-conjugated IgG1 antibody (Clone NO.: YA3442), targeting MDM2, with a predicted molecular weight of 55 kDa (observed band size: 90 kDa). MDM2 Antibody (YA3442) can be used for WB, ICC/IF, IHC-P experiment in human, monkey background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

MDM2 Antibody (YA3442) is a mouse-derived non-conjugated IgG1 antibody (Clone NO.: YA3442), targeting MDM2, with a predicted molecular weight of 55 kDa (observed band size: 90 kDa). MDM2 Antibody (YA3442) can be used for WB, ICC/IF, IHC-P experiment in human, monkey background.
Gene ID
SwissProt ID
Synonyms
ACTFS antibody; Double minute 2 protein antibody; E3 ubiquitin-protein ligase Mdm2 antibody; Hdm 2 antibody; Hdm2 antibody; HDMX antibody; MDM 2 antibody; MDM2 antibody; MDM2 oncogene E3 ubiquitin protein ligase antibody; Mdm2 p53 E3 ubiquitin protein ligase homolog antibody; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody; MDM2_HUMAN antibody; MDM2BP antibody; Mouse Double Minute 2 antibody; MTBP antibody; Murine Double Minute Chromosome 2 antibody; Oncoprotein Mdm2 antibody; p53 Binding Protein Mdm2 antibody; p53-binding protein Mdm2 antibody; Ubiquitin protein ligase E3 Mdm2 antibody; Ubiquitin protein ligase E3 Mdm2 antibody
Molecular Weight

Predicted band size: 55 kDa;Observed band size: 90 kDa
Purity

Affinity Purified
Subcellular Location

Nucleus, nucleoplasm, nucleolus, Cytoplasm.
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Product Categories

Primary Antibody; Recombinant Mouse Monoclonal Antibody
Clonality

Monoclonal, Recombinant
Host

Mouse
Reactivity

Human, Monkey
Dilution Ratio

WB: 1:1,000;IF-Cell: 1:500;IHC-P: 1:1,000

  • Western blot analysis of extracts from HepG2(lane2(20μg),HepG2(lane 3(40μg) , using MDM2 Antibody (HY-P80705) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application

WB, IHC-P, ICC/IF
Positive Control

U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate, HepG2 cell lysate, COS-1cell lysate, U-2 OS cells treated with 10μM NUTLIN 3A for 24 hours, human embryonal carcinoma tissue, human te
Appearance

Liquid
Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG1
Immunogen

Recombinant protein within human MDM2 aa 1-491 / 491.
Database

Entrez Gene: 4193 Human ; 17246 Mouse ; 314856 Rat

SwissProt: Q00987 Human ; P23804 Mouse ;

OMIM: 618681 Human

Documentation

COA (205 KB)

User Guide for Antibodies (1077 KB)

MDM2 Antibody (YA3442) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MDM2 Antibody (YA3442)
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HY-P83705
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