MDM2 Antibody (YA3442)

MDM2 Antibody (YA3442) is a mouse-derived non-conjugated IgG1 antibody (Clone NO.: YA3442), targeting MDM2, with a predicted molecular weight of 55 kDa (observed band size: 90 kDa). MDM2 Antibody (YA3442) can be used for WB, ICC/IF, IHC-P experiment in human, monkey background.

MDM2 Protein, an E3 ubiquitin-protein ligase, plays a central role in the intricate regulation of cellular processes by mediating the ubiquitination and subsequent proteasomal degradation of the tumor suppressor p53/TP53, leading to its downregulation. Beyond its interaction with p53/TP53, MDM2 inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding to their transcriptional activation domain, highlighting its role as a negative regulator of these critical cellular responses. Not limited to its interactions with p53/TP53, MDM2 functions as a ubiquitin ligase E3 toward itself and ARRB1, permitting the nuclear export of p53/TP53 and promoting the proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Additionally, MDM2 inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation, demonstrating its versatile involvement in apoptotic pathways. As a component of various complexes, including TRIM28/KAP1-MDM2-p53/TP53 and TRIM28/KAP1-ERBB4-MDM2, MDM2 links growth factor and DNA damage response pathways. It further mediates ubiquitination and proteasome degradation of DYRK2, IGF1R, SNAI1, DCX, and DLG4, influencing diverse cellular functions such as dendritic spine density, receptor endocytosis, and mitochondrial respiration. Through its interactions with NDUFS1, MDM2 negatively regulates mitochondrial respiration, leading to increased oxidative stress and commitment to the mitochondrial pathway of apoptosis. These multifaceted roles underscore the intricate regulatory functions of MDM2 in cellular homeostasis and stress responses.

Q00987

Predicted band size: 55 kDa;Observed band size: 90 kDa

Affinity Purified

Nucleus, nucleoplasm, nucleolus, Cytoplasm.

Non-conjugated

Unmodified

AB_3676587

Primary Antibody; Recombinant Mouse Monoclonal Antibody

Monoclonal, Recombinant

Mouse

Human, Monkey

WB: 1:1,000;IF-Cell: 1:500;IHC-P: 1:1,000

• 
  Western blot analysis of extracts from HepG2(lane2(20μg),HepG2(lane 3(40μg) , using MDM2 Antibody (HY-P80705) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of MCF-7 cells labeling MDM2 with MDM2 Antibody (HY-P83705) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MDM2 Antibody (HY-P83705) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling MDM2 with MDM2 Antibody (HY-P83705) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MDM2 Antibody (HY-P83705)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF

U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate, HepG2 cell lysate, COS-1cell lysate, U-2 OS cells treated with 10μM NUTLIN 3A for 24 hours, human embryonal carcinoma tissue, hum

Liquid

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Recombinant protein within human MDM2 aa 1-491 / 491.