MCM2 Antibody (YA302)

MCM2 Antibody (YA302) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM2.

MCM2 is a core component of the MCM2-7 complex (MCM complex), the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. It forms part of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and serves as the scaffold for replisome assembly (by similar: PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The ATPase active sites in the MCM2-7 ring are generated through interfaces of adjacent subunits, with a conserved arginine finger motif acting in trans on the Walker A box ATP-binding site of neighboring subunits, though the six ATPase sites likely contribute asymmetrically to helicase activity (by similar: PubMed:32453425). MCM2 is required for S-phase entry and cell division (by similar: PubMed:8175912) and also regulates terminally differentiated cochlear hair cell development and apoptosis induction (by similar: PubMed:26196677).

Free

P49736

Predicted band size: 102 kDa；Observed band size: 130 kDa

Protein A affinity purified.

Nucleus, Chromosome.

Non-conjugated

Unmodified

AB_3102861

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:2,000 ;IHC-P: 1:800-1:2,000 ;ICC: 1:50 ;FC: 1:500-1:1,000

• 
  Western blot analysis of extracts from Si-ha(lane 2(20μg) and SiHa(lane 3(40μg) using MCM2(HY-P80215 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of MCF-7 cells labeling MCM2 with MCM2 Antibody (HY-P80215) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCM2 Antibody (HY-P80215) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling MCM2 with MCM2 Antibody (HY-P80215) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MCM2 Antibody (HY-P80215)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein within