ERK1 Antibody (YA3921)

ERK1 Antibody (YA3921) is a mouse-derived and non-conjugated IgG1 antibody, targeting to MAPK3. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, monkey.

ERK1, a serine/threonine kinase, stands as a vital component in the MAP kinase signal transduction pathway, working in concert with MAPK1/ERK2 to orchestrate the MAPK/ERK cascade. This pathway, activated by stimuli such as KIT and KITLG/SCF, plays a pivotal role in diverse biological functions, including cell growth, adhesion, survival, and differentiation. ERK1, along with MAPK1/ERK2, influences transcription, translation, and cytoskeletal rearrangements, while also contributing to the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells through the phosphorylation of numerous transcription factors. With approximately 160 substrates identified for ERKs, their targets span various cellular compartments, participating in processes such as transcription regulation, translation, mitosis, and apoptosis. Additionally, the MAPK/ERK cascade is implicated in endosomal dynamics, lysosome processing, endosome cycling through the perinuclear recycling compartment (PNRC), and the fragmentation of the Golgi apparatus during mitosis. Noteworthy substrates include transcription factors, cytoskeletal elements, regulators of apoptosis, regulators of translation, as well as diverse signaling-related molecules, protein kinases, and phosphatases, thereby extending the cascade's specificity and amplifying its impact on cellular responses.

P27361

Predicted band size: 43 kDa; Observed band size: 43 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

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  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using MAPK3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human MAPK3 expressed in E. Coli.