p38 alpha/MAPK14 Antibody (YA706)

p38 alpha/MAPK14 Antibody (YA706) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to p38 alpha/MAPK14.

MAPK14 is a serine/threonine kinase that serves as a core component of the MAP kinase signaling pathway. As one of the four p38 MAPKs, it plays a pivotal role in cellular response cascades triggered by extracellular stimuli like pro-inflammatory cytokines or physical stress, directly activating transcription factors. p38 MAPKs phosphorylate approximately 200-300 substrates, including downstream kinases (e.g., RPS6KA5/MSK1 and RPS6KA4/MSK2, which phosphorylate transcription factors such as CREB1, ATF1, RELA/NFKB3, STAT1, STAT3, as well as histone H3 and nucleosomal protein HMGN1). Other kinase targets like MAPKAPK2/MK2 and MAPKAPK3/MK3 regulate post-transcriptional gene expression by phosphorylating ZFP36 and ELAVL1. by similar: MAPK14 also activates MKNK1/MNK1 and MKNK2/MNK2 to phosphorylate translation initiation factor EIF4E2, and interacts with casein kinase II to activate TP53/p53. In the cytoplasm, it regulates protein degradation via phosphorylating CFLAR and SIAH2, while inhibiting autophagy by disrupting ATG9 trafficking. Additionally, MAPK14 controls receptor endocytosis by phosphorylating EGFR and RAB5A effectors, and activates ADAM17-mediated TGF-α shedding to stimulate EGFR signaling. In the nucleus, it phosphorylates transcription factors (e.g., ATF2, ELK1, TP53) and enhances chromatin accessibility of inflammatory genes (e.g., IL6, IL8) via histone H3 Ser-10 phosphorylation (H3S10ph). Kinase-independent roles include modulating O-GlcNAcylation through OGT interaction. During development, MAPK14 regulates EPO expression for placental angiogenesis and stress erythropoiesis. Isoform MXI2 is activated by oxidative stress, while EXIP may initiate apoptosis. During microbial infection, M.tuberculosis EsxA activates MAPK14 in T-cells to suppress IFN-γ production.

Free

Q16539

Predicted band size: 41 kDa; Observed band size: 38 kDa

Protein A affinity purified.

Cytoplasm, nucleus

Non-conjugated

Unmodified

AB_3102353

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:500 ;ICC: 1:100-1:200 ;IHC-P: 1:100-1:200

• 
  Western blot analysis of extracts from HepG2 (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , and HEK293T (lane 4(20μg)using MAPK14 (HY-P80213) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80213, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of RAW264.7 cells labeling MAPK14 with MAPK14 Antibody (HY-P80213) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAPK14 Antibody (HY-P80213)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling MAPK14 with MAPK14 Antibody (HY-P80213) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAPK14 Antibody (HY-P80213) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide corresponding to Human MAPK14.AA range:1-50.