Lipocalin 2 Antibody (YA992)

Lipocalin 2 Antibody (YA992) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lipocalin 2.

The related gene encodes a protein that belongs to the lipocalin family. Members of The family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by The related gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of The protein in blood and urine is an early biomarker of acute kidney injury. The protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking The related gene are more susceptible to bacterial infection than wild type mice.

P80188

Predicted band size: 23 kDa; Observed band size: 23 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3103125

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

• 
  Immunocytochemistry analysis of A549 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of A431 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Lipocalin-2