LC3A/B Antibody

Cat. No.: HY-P80741: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

LC3A/B Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LC3A/B.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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LC3A/B Antibody Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
Technical Parameters
Properties
Documentation

Description	LC3A/B Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LC3A/B.
Background	MAP1LC3A, a ubiquitin-like modifier, plays a crucial role in autophagosomal vacuole formation, contributing to cellular homeostasis. While participating in the elongation of the phagophore membrane, MAP1LC3A, belonging to the GABARAP/GATE-16 subfamily, assumes a vital role in the later stages of autophagosome maturation. It engages in the remodeling of endoplasmic reticulum subdomains into autophagosomes in response to nutrient stress, facilitating their subsequent fusion with lysosomes for endoplasmic reticulum turnover. With three different light chains (LC1, LC2, and LC3), it can associate with MAP1A and MAP1B proteins. MAP1LC3A exhibits a diverse interactome, engaging with proteins like TP53INP1, TP53INP2, SQSTM1 for inclusion body degradation, ATG13, ULK1, TBC1D5, UBQLN1, UBQLN2, UBQLN4, TRIM5, MEFV, and others. These interactions underscore the multifaceted role of MAP1LC3A in various cellular processes, including autophagy, protein aggregate degradation, and the regulation of reticulophagy.
Tag	Free
Gene ID	81631 [NCBI]
SwissProt ID	Q9H492 / Q9GZQ8
Synonyms	LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B
Molecular Weight	Predicted band size: 14 kDa; Observed band size: 14/16 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3102687
Research Field	Signal Transduction
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1:500-1:1000 Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg) and C2C12(lane 4(20μg) using LC3A/B (HY-P80741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application	WB
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Database	Entrez Gene: 81631 Human ; 84557 Human ; 66734 Mouse ; 67443 Mouse ; 362245 Rat ; 64862 Rat SwissProt: Q9GZQ8 Human ; Q9H492 Human ; Q91VR7 Mouse ; Q9CQV6 Mouse ; Q62625 Rat Q6XVN8 OMIM: 609604 Human
Documentation	Select Batch: Data Sheet (235 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

LC3A/B Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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LC3A/B Antibody

LC3A/B Antibody Featured Recommendations:

Biological Activity

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LC3A/B Antibody Related Classifications

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