LAMP2 Antibody (YA309)

LAMP2 Antibody (YA309) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LAMP2.

CD107b is a lysosomal membrane glycoprotein that plays a crucial role in lysosome biogenesis, lysosomal pH regulation, and autophagy. It acts as a key regulator of lysosomal lumen pH by directly inhibiting the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. CD107b is essential for chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins under stress conditions and during the normal turnover of long-lived proteins. It functions by binding target proteins such as GAPDH, NLRP3, and MLLT11, directing them for lysosomal degradation. In chaperone-mediated autophagy, CD107b operates downstream of chaperones like HSPA8/HSC70, which recognize and recruit substrate proteins to lysosomes, where they bind to LAMP2. Additionally, CD107b is involved in lysosomal protein degradation during starvation (by similarity) and mediates the fusion of autophagosomes with lysosomes during autophagy. Cells lacking LAMP2 fail to accumulate STX17 on autophagosomes, likely explaining the impaired fusion process. CD107b also contributes to MHC class II-mediated presentation of exogenous antigens by facilitating lysosomal protein degradation, generating antigenic peptides captured by MHC II subunits. However, it is not required for the presentation of endogenous antigens. Furthermore, CD107b supports FURIN-mediated cleavage of the mumps virus fusion protein F by interacting with both FURIN and the unprocessed form of the viral protein F.

Free

P13473

Predicted band size: 45 kDa;Observed band size: 70-140 kDa

Protein A affinity purified.

Cell membrane, Endosome membrane, Lysosome membrane

Non-conjugated

Unmodified

AB_3102351

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500 ;IHC-P: 1:50-1:500 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from NIH/3T3 (lane 2(20μg) ) and NIH/3T3 (lane 3(40μg) )using LAMP2a (HY-P80208) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80208, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using LAMP2a Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80208, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using LAMP2a Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80208, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human LAMP2a.AA range:361-410.