LAMP1 Antibody (YA3434)

LAMP1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 45 kDa, targeting to LAMP1. It can be used for WB, IHC-P, IF-Tissue, IHC-Fr, IP assays with tag free, in the background of Human,Mouse,Rat.

LAMP1 is a lysosomal membrane glycoprotein that plays crucial roles in lysosome biogenesis, lysosomal pH regulation, autophagy, and cholesterol homeostasis. It acts as a key regulator of lysosomal lumen pH by directly inhibiting the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. LAMP1 is also essential for NK-cell cytotoxicity, participating in cytotoxic granule movement to the cell surface and perforin trafficking to lytic granules, while protecting NK-cells from degranulation-associated damage caused by their own cytotoxic granule content. Additionally, LAMP1 presents carbohydrate ligands to selectins. In microbial infections, it serves as a receptor for the Lassa virus glycoprotein and promotes virus-host membrane fusion in less acidic endosomes. By similar function, LAMP1 supports FURIN-mediated cleavage of the mumps virus fusion protein F by interacting with both FURIN and the unprocessed form of the viral protein F.

P11279

Predicted band size: 45 kDa;Observed band size: 100-120 kDa

Protein A affinity purified.

Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane.

Non-conjugated

Unmodified

AB_3694231

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:2,000; IHC-P: 1:10,000; IF-Tissue: 1:500-1:2,000; IHC-Fr: 1:1,000; IP: 1-2μg/500μL sample

• 
  Western blot analysis of extracts from MDA-MB231 (lane 2(20μg) and NIH3T3 (lane 3(20μg) using LAMP1 (HY-P80206A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using LAMP1 Antibody (YA3434). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80206A, 1/10000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using LAMP1 Antibody (YA3434). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80206A, 1/10000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunocytochemistry analysis of Neuro-2a cells labeling LAMP1 with LAMP1 Antibody (HY-P80206A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LAMP1Antibody (HY-P80206A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling LAMP1with LAMP1 Antibody (HY-P80206A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LAMP1Antibody (HY-P80206A)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, IHC-F, ICC/IF, IP, IF-Tissue

Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, human kidney tissue, mouse colon tissue, m

Liquid

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within mouse LAMP1 aa 371-406.