Ku70 Antibody (YA715)

Ku70 Antibody (YA715) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Ku70.

Ku70 is a single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA, participating in double-strand break repair and V(D)J recombination. It is also involved in chromosome translocation, preferentially binding to fork-like ends of double-stranded DNA in a cell cycle-dependent manner and working in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer recognizes and binds to broken DNA ends, protecting them from further resection, and regulates DNA-PK complex activity by increasing PRKDC's affinity to DNA 100-fold. Ku70 may also function as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), cleaning abasic site termini. It acts as a negative transcriptional regulator with APEX1 and activates osteocalcin expression in association with NAA15. Additionally, Ku70 modulates DNA virus-mediated innate immune response by forming the HDP-RNP complex and suppresses apoptosis by interacting with BAX.

Free

P12956

Predicted band size: 70 kDa;Observed band size: 75 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102855

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:2000; IHC-P: 1:100-1:200

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), A549 (lane 3(20μg) and HEK293(lane 4(20μg) using Ku70 (HY-P80202) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Ku70 Antibody (YA715). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80202, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Ku70 Antibody (YA715). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80202, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide corresponding to Human Ku70.AA range:1-150.