c-Kit Antibody (YA3747)

c-Kit Antibody (YA3747) is a mouse-derived and non-conjugated IgG1 antibody, targeting to KIT. It can be applicated for WB, IHC-P, ELISA assays, in the background of human.

c-Kit is a tyrosine-protein kinase that functions as a cell-surface receptor for the cytokine KITLG/SCF, playing a vital role in regulating cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development/function, and melanogenesis. Upon KITLG/SCF binding, c-Kit activates multiple signaling pathways, phosphorylating PIK3R1, PLCG1, SH2B2/APS, and CBL. It triggers the AKT1 pathway via PIK3R1 phosphorylation (the regulatory subunit of phosphatidylinositol 3-kinase) and transmits signals through GRB2 to activate RAS, RAF1, and MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. c-Kit also promotes STAT family member (STAT1/3/5A/5B) activation and PLCG1-mediated production of diacylglycerol and inositol 1,4,5-trisphosphate. Its signaling is modulated by protein phosphatases and receptor internalization/degradation, while activated c-Kit induces phosphorylation of PTPN6/SHP-1, PTPRU, STAT1/3/5A/5B, PIK3R1, CBL, CRK (Crk-II isoform), LYN, MAPK1/ERK2/ERK1, PLCG1, SRC, and SHC1.

P10721

Predicted band size: 110 kDa; Observed band size: 145 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; ELISA: 1/10000

WB, IHC-P, ELISA

Liquid

Supplied in ascitic fluid containing 0.03% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human KIT expressed in E. Coli.