JNK1+JNK3 Antibody (YA327)

JNK1+JNK3 Antibody (YA327) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JNK1+JNK3.

JNK1, a serine/threonine-protein kinase, intricately regulates diverse cellular processes, including cell proliferation, differentiation, migration, transformation, and programmed cell death. Activation of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, stimulated by extracellular factors such as pro-inflammatory cytokines or physical stress, involves the phosphorylation and activation of JNK1 by the dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7. In the ensuing cascade, JNK1 phosphorylates key transcription factors, particularly components of AP-1 such as JUN, JDP2, and ATF2, thereby modulating AP-1 transcriptional activity. JNK1 also exerts regulatory control over replication initiation by phosphorylating the replication licensing factor CDT1, disrupting its interaction with the histone H4 acetylase HBO1. This event impedes the acetylation required for replication origins. Additionally, JNK1 plays a pivotal role in apoptosis induction by phosphorylating critical regulatory factors, including p53/TP53 and Yes-associates protein YAP1. Its involvement in diverse cellular pathways encompasses contributions to T-cell differentiation, survival of erythroid cells, autophagy activation, and regulation of microtubule dynamics in neurons. JNK1's extensive substrate repertoire includes heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, the E3 ligase ITCH, the circadian clock regulator CLOCK-BMAL1, and the heat shock transcription factor HSF1, among others. Notably, JNK1 isoforms exhibit distinct binding patterns, with beta-1 preferentially binding to c-Jun, while alpha-1, alpha-2, and beta-2 display a similar low level of binding to both c-Jun and ATF2, although phosphorylation efficiency remains consistent across isoforms.

Free

P45983

Predicted band size: 48/53 kDa; Observed band size: 48/53 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Membrane, Mitochondrion.

Non-conjugated

Unmodified

AB_3102343

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Mouse, Rat

WB: 1:1,000-1:2,000 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg), NIH3T3(lane 4(20μg)) and NIH3T3(lane 5(40μg)) using JNK1+JNK3 (HY-P80198) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80198, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human JNK1 + JNK3.AA range:1-423.