IRS-1 Antibody (YA5813)

IRS-1 Antibody (YA5813) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRS-1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

IRS-1 is a signaling adapter protein that mediates signal transduction from the insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R). It plays a critical role in development, growth, glucose homeostasis, and lipid metabolism. Upon phosphorylation by the insulin receptor, IRS-1 acts as a signaling scaffold, propagating insulin action by binding to SH2 domain-containing proteins such as the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2, or SHP2. Recruitment of GRB2 activates the guanine nucleotide exchange factor SOS1, triggering the Ras/Raf/MEK/MAPK signaling cascade (by similar). The PI3K/AKT pathway, activated by IRS-1, mediates most of insulin's metabolic effects, while the Ras/Raf/MEK/MAPK pathway regulates gene expression and, in coordination with PI3K, controls cell growth and differentiation. Additionally, IRS-1 positively regulates the Wnt/β-catenin signaling pathway by suppressing autophagy-mediated degradation of DVL2, thereby promoting cell proliferation.

P35568

Predicted band size: 131 kDa; Observed band size: 180 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:400-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG