Insulin Receptor Beta Antibody (YA341)

Insulin Receptor Beta Antibody (YA341) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Insulin Receptor Beta.

CD220 is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Insulin binding triggers phosphorylation of intracellular substrates such as insulin receptor substrates (IRS1, IRS2, IRS3, IRS4), SHC, GAB1, CBL, and other signaling intermediates. These phosphorylated proteins serve as docking platforms for SH2 domain-containing signaling proteins like the p85 regulatory subunit of PI3K and SHP2, activating two major pathways: the PI3K-AKT/PKB pathway (responsible for most metabolic effects of insulin) and the Ras-MAPK pathway (regulating gene expression and cooperating with PI3K to control cell growth/differentiation). PI3K recruitment to phospho-IRS1 generates phosphatidylinositol-(3,4,5)-trisphosphate (PIP3), activating PDPK1 and AKT/PKB to promote GLUT4 translocation for glucose uptake. Activated AKT also exerts anti-apoptotic effects via BAD phosphorylation and regulates gluconeogenic/lipogenic enzymes through FOX transcription factors. The PI3K-AKT axis additionally modulates mTORC1 signaling to integrate insulin-dependent growth/metabolic responses, with AKT phosphorylating TSC2 to activate mTORC1-mediated protein synthesis. The Ras/RAF/MAP2K/MAPK cascade, initiated by GRB2/SOS recruitment to phospho-IRS1, primarily mediates insulin's mitogenic/differentiation effects. Beyond insulin, CD220 binds IGF1/IGF2, with the Short isoform showing higher IGF2 affinity. Hybrid receptors with IGF1R exhibit variant ligand specificity: IGF1R-Long hybrids respond strongly to IGF1 but weakly to IGF2/insulin, while IGF1R-Short hybrids are activated by all three ligands (though some studies report similar IGF1 preference and low insulin affinity for both hybrids). In adipocytes, it suppresses lipolysis (by similar).

Free

P06213

Predicted band size: 156 kDa;Observed band size: 95 kDa

Protein A affinity purified.

Cell membrane.

Non-conjugated

Unmodified

AB_3102338

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500-1:2,000 ;ICC/IF: 1:100-1:500

• 
  Western blot analysis of extracts from HCT116 (lane 2(20μg)，HepG2 (lane 3(20μg) and MDA-MB231 (lane 4(20μg) using Insulin Receptor Beta (HY-P80192) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling Insulin Receptor Beta with Insulin Receptor Beta Antibody (HY-P80192)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Insulin Receptor Beta Antibody (HY-P80192) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling Insulin Receptor Beta with Insulin Receptor Beta Antibody (HY-P80192) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Insulin Receptor Beta Antibody (HY-P80192) at 1/250 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Insulin Receptor.AA range:930-970.