IKK gamma Antibody (YA346)

IKK gamma Antibody (YA346) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK gamma.

IKK gamma is the regulatory subunit of the IKK core complex, which phosphorylates inhibitors of NF-kappa-B, leading to the dissociation and degradation of the inhibitor/NF-kappa-B complex. Its binding to scaffolding polyubiquitin is crucial for IKK activation by multiple signaling receptor pathways. IKK gamma can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a higher affinity for linear polyubiquitin. It may also play a role in NF-kappa-B-mediated protection from cytokine toxicity and is essential for viral activation of IRF3. Additionally, it is involved in the TLR3- and IFIH1-mediated antiviral innate immune response, requiring 'Lys-27'-linked polyubiquitination. Similar functions include serving as a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B.

Free

Q9Y6K9

Predicted band size: 48 kDa; Observed band size: 48 kDa

Protein A affinity purified.

Nucleus. Cytoplasm.

Non-conjugated

Unmodified

AB_3102499

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:400; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using IKK gamma antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80415, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using IKK gamma antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80415, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using IKK gamma antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80415, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using IKK gamma antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80415, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

WB, IHC-P, ICC/IF, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human IKK gamma.AA range:1-286.