IKK alpha Antibody (YA348)

IKK alpha Antibody (YA348) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK alpha.

CHUK is a serine kinase that plays a pivotal role in the NF-kappa-B signaling pathway, which is activated by various stimuli including inflammatory cytokines, bacterial or viral products, and DNA damage. As part of the canonical IKK complex, CHUK phosphorylates NF-kappa-B inhibitors on serine residues, leading to their ubiquitination and subsequent proteasomal degradation, thereby releasing NF-kappa-B to translocate into the nucleus and activate the transcription of hundreds of genes involved in immune response, growth control, or anti-apoptosis. Additionally, CHUK negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 to promote the assembly of the A20/TNFAIP3 ubiquitin-editing complex, limiting inflammatory gene activation. In the non-canonical NF-kappa-B pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes that regulate B-cell survival and lymphoid organogenesis. CHUK also participates in the negative feedback of this pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, CHUK phosphorylates CREBBP to enhance its transcriptional and histone acetyltransferase activities, and modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histone H3 at Ser-10, which is subsequently acetylated at Lys-14 by CREBBP. Furthermore, CHUK phosphorylates the CREBBP-interacting protein NCOA3 and the pro-apoptotic transcription factor FOXO3. By similarity, CHUK phosphorylates RIPK1 at Ser-25 to repress its kinase activity, preventing TNF-mediated RIPK1-dependent cell death. Following mitophagy induction, CHUK phosphorylates AMBRA1 to promote its interaction with ATG8 family proteins and enhance its mitophagic activity.

Free

O15111

Predicted band size: 85 kDa;Observed band size: 85 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102497

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from RAW264.7(lane 2(20μg) or lane 3(40μg)) and C6(lane 4(20μg) or lane 5(40μg)), using IKK alpha (HY-P80413) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80413, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P, FC, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within N-terminal human IKK alpha.