Histone H3 (tri methyl K9) Antibody (YA734)

Cat. No.: HY-P80176: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Histone H3 (tri methyl K9) Antibody (YA734) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (tri methyl K9).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Histone H3 (tri methyl K9) Antibody (YA734) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Histone H3 (tri methyl K9) Antibody (YA734) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (tri methyl K9).

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 15 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P68431

Gene ID

8350 [NCBI]

Immunogen

Synthetic peptide within N terminal human Histone H3 including tri methyl K9 conjugated to keyhole limpet haemocyanin.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200

Sensitivity	Endogenous	Purity	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Methylated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HCT116(lane 2(20μg) and HCT116(lane 3(40μg) using Histone H3 (tri methyl K9) (HY-P80176) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80176, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

RRID

AB_3102329

Database

Entrez Gene: 8350 Human ; 319152 Mouse ; 291159 Rat

SwissProt: P68431 Human ; P68433 Mouse ; Q6LED0 Rat

OMIM: 137800 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

H3K9me3; H3 histone family member E pseudogene; H3 histone family, memberA; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; HistoneH3/l; H3K9me2; HistoneH3(trimethylK9)

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Histone H3 (tri methyl K9) Antibody (YA734) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Histone H3 (tri methyl K9) Antibody (YA734)

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