Histone H2B Antibody (YA378)

Cat. No.: HY-P80163: Data Sheet COA
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Histone H2B Antibody (YA378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H2B.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Histone H2B Antibody (YA378) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	Histone H2B Antibody (YA378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H2B.
Background	Histone H2B is a core component of nucleosomes, which wrap and compact DNA into chromatin, thereby limiting DNA accessibility to cellular machineries that require DNA as a template. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and nucleosome remodeling. Additionally, Histone H2B exhibits broad antibacterial activity and may contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, as well as the bactericidal activity of amniotic fluid.
Tag	Free
Gene ID	337874 [NCBI]
SwissProt ID	O60814
Synonyms	H2B GL105 antibody; H2B histone family member O antibody; H2B histone family member S antibody; H2B.1 antibody; H2B.1 B antibody; H2B.b antibody; H2B.c antibody; H2B.d antibody; H2B.e antibody; H2B.f antibody; H2B.j antibody; H2B.q antibody; H2B/b antibody; H2B/c antibody; H2B/d antibody; H2B/e antibody; H2B/f antibody; H2B/j antibody; H2B/o antibody; H2B/q antibody; H2BFB antibody; H2BFC antibody; H2BFD antibody; H2BFE antibody; H2BFF antibody; H2BFJ antibody; H2BFO antibody; H2BFQ antibody; H2BFS antibody; HIRIP2 antibody; HIST1H2BB antibody; HIST1H2BD antibody; HIST1H2BH antibody; HIST1H2BL antibody; HIST1H2BM antibody; HIST1H2BN antibody; HIST2H2BE antibody; Histone H2B antibody; Histone H2B type 1 B antibody; Histone H2B type 1 D antibody; Histone H2B type 1 H antibody; Histone H2B type 1 L antibody; Histone H2B type 1 M antibody; Histone H2B type 1 N antibody; Histone H2B type 2 E antibody; histone protein antibody
Molecular Weight	Predicted band size: 14 kDa; Observed band size: 14 kDa
Purity	Protein A affinity purified.
Subcellular Location	Nucleus, Chromosome.
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3102324
Research Field	Epigenetics and Nuclear Signaling
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1:500-1:2,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:5,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80163, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)using Histone H2B (HY-P80163) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80163, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80163, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunocytochemistry analysis of A431 cells labeling Histone H2B with Histone H2B antibody (HY-P80163) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H2B antibody (HY-P80163) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Western blot analysis of extracts from Hela (lane 2(20μg) , Hela (lane 3(20μg),using Histone H2B Antibody (HY-P80163). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application	WB, ICC/IF, IHC-P, IP, FC
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human Histone H2B.AA range:110 to the C-terminus .
Database	Entrez Gene: 337874 Human ; 54145 Human ; 8349 Human ; 104021 Mouse ; 64647 Rat SwissProt: O60814 Human ; P06899 Human ; P23527 Human ; P33778 Human ; P57053 Human ; P58876 Human ; P62807 Human ; Q16778 Human ; Q5QNW6 Human ; Q8N257 Human ; Q93079 Human ; Q99877 Human ; Q99879 Human ; Q99880 Human ; P10854 Mouse ; Q64475 Mouse ; Q00729 Rat OMIM: 615045 Human
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Histone H2B Antibody (YA378) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Histone H2B Antibody (YA378)

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