His Tag Antibody (HRP) (YA877)

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

Predicted band size: 1 kDa; Observed band size: 47 kDa

Affinity Purified

HRP

Unmodified

Tags & Cell Markers

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Species independent

WB: 1:1000-1:10000

• 
  Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

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