His Tag Antibody (HRP) (YA877)

Cat. No.: HY-P80947A: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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His Tag Antibody (HRP) (YA877) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 1 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Species independent

Immunogen

HHHHHH

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	HRP	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Research Field

Tags & Cell Markers

Documentation

Select Batch:

Data Sheet (230 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

His Tag Antibody (HRP) (YA877) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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His Tag Antibody (HRP) (YA877)

His Tag Antibody (HRP) (YA877) Featured Recommendations:

Product Detail

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Background

Documentation

His Tag Antibody (HRP) (YA877) Related Classifications

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