HDAC8 Antibody (YA2994)

HDAC8 Antibody (YA2994) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2994), targeting HDAC8, with a predicted molecular weight of 42 kDa (observed band size: 42 kDa). HDAC8 Antibody (YA2994) can be used for WB, IP experiment in human background.

HDAC8 Protein, a histone deacetylase, catalyzes the deacetylation of lysine residues located on the N-terminal region of core histones, namely H2A, H2B, H3, and H4, as supported by various studies. This enzymatic activity is crucial in initiating epigenetic repression, playing pivotal roles in transcriptional regulation, cell cycle progression, and developmental events. Acting within large multiprotein complexes, histone deacetylases like HDAC8 contribute to the intricate regulation of chromatin structure and function. Moreover, HDAC8 is implicated in the deacetylation of the cohesin complex protein SMC3, influencing the release of cohesin complexes from chromatin. Notably, HDAC8 may also play a role in smooth muscle cell contractility. Expanding its enzymatic repertoire, HDAC8 exhibits protein-lysine deacylase activity, specifically functioning as a protein decrotonylase by mediating the decrotonylation of histones, particularly crotonylation at lysine residues ((2E)-butenoyl).

Q9BY41

Predicted band size: 42 kDa; Observed band size: 42 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105363

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IP: 1/20

• 
  Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using HDAC8 Antibody (HY-P83249) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human HDAC8