HDAC6 Antibody (YA390)

HDAC6 Antibody (YA390) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC6.

The HDAC6 protein assumes a multifaceted role, primarily responsible for the deacetylation of lysine residues on the N-terminal portion of core histones (H2A, H2B, H3, and H4), contributing to the epigenetic repression that plays crucial roles in transcriptional regulation, cell cycle progression, and developmental events. Operating within large multiprotein complexes, histone deacetylases, including HDAC6, orchestrate these processes. Beyond histones, HDAC6 extends its deacetylation activity to diverse proteins, such as tubulin, essential for microtubule-dependent cell motility, and alpha-tubulin, pivotal for cilia disassembly. Additionally, HDAC6 facilitates actin polymerization and autophagosome-lysosome fusion by deacetylating CTTN, promoting autophagy completion. Its involvement in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer and promotion of odontoblast differentiation showcase its diverse functions. Furthermore, HDAC6 plays a key role in the degradation of misfolded proteins, acting as an adapter to recognize polyubiquitinated misfolded proteins and targeting them to the aggresome for clearance via autophagy when the chaperone refolding system and the ubiquitin-proteasome are insufficient.

Free

Q9UBN7

Predicted band size: 131 kDa; Observed band size: 160 kDa

Protein A affinity purified.

Cell projection, Cytoplasm, Cytoskeleton, Nucleus.

Non-conjugated

Unmodified

AB_3102320

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000-1:5,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:400; IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using HDAC6 Antibody (HY-P80154) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling HDAC6 Antibody (HY-P80154) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC6 Antibody (HY-P80154) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling HDAC6 Antibody (HY-P80154) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC6 Antibody (HY-P80154) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC6.AA range:1-50.