HDAC2 Antibody (YA393)

HDAC2 Antibody (YA393) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC2.

HDAC2 is a histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal regions of core histones (H2A, H2B, H3, and H4). Histone deacetylation serves as an epigenetic repression marker and plays a key role in transcriptional regulation, cell cycle progression, and developmental events (by similar). It functions by forming large multiprotein complexes (by similar) and associates with MAD, SIN3, YY1, and N-COR to establish transcriptional repressor complexes. As part of the RCOR/GFI/KDM1A/HDAC complex, HDAC2 suppresses genes involved in multilineage blood cell development via histone deacetylase recruitment (by similar). It is also a component of the histone deacetylase NuRD complex, which participates in chromatin remodeling. Additionally, HDAC2 contributes to the SIN3B complex, repressing transcription and counteracting EP300's histone acetyltransferase activity by recognizing H3K27ac marks via PHF12 and leveraging its deacetylase function. Beyond histones, HDAC2 deacetylates non-histone targets like TSHZ3, modulating its transcriptional repressor activity. It may also be involved in CRY1-mediated transcriptional repression of circadian genes such as PER1 through histone deacetylation (by similar). Furthermore, HDAC2 exhibits protein-lysine deacylase activity, removing crotonyl, lactyl, and 2-hydroxyisobutyryl groups from lysine residues, leading to protein decrotonylation, delactylation, and de-2-hydroxyisobutyrylation.

Free

Q92769

Predicted band size: 55 kDa; Observed band size: 55 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102096

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Immunocytochemistry analysis of NIH3T3 cells labeling HDAC2 with HDAC2 Antibody (HY-P80151) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC2 Antibody (HY-P80151) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling HDAC2 with HDAC2 Antibody (HY-P80151) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC2 Antibody (HY-P80151)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using HDAC2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using HDAC2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC2.AA range:439-488.