HDAC1 Antibody (YA395)

HDAC1 Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to HDAC1. It can be used as a loading control antibody.

HDAC1 is a histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of core histones (H2A, H2B, H3, and H4). Histone deacetylation serves as an epigenetic repression tag and plays a critical role in transcriptional regulation, cell cycle progression, and developmental events. HDAC1 functions by forming large multiprotein complexes and is a component of the NuRD histone deacetylase complex, which participates in chromatin remodeling. As part of the SIN3B complex, it is recruited downstream of constitutively active gene transcription start sites through histone interactions, mitigating histone acetylation and RNA polymerase II progression within transcribed regions to regulate transcription. HDAC1 also acts as a deacetylase for non-histone targets, including NR1D2, RELA, SP1, SP3, STAT3, and TSHZ3. It deacetylates SP proteins (SP1 and SP3) to regulate their function. Additionally, HDAC1 is a component of the BRG1-RB1-HDAC1 complex, which negatively regulates CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex, allowing CREBBP recruitment to facilitate transcriptional activation. HDAC1 deacetylates TSHZ3 to modulate its transcriptional repressor activity, deacetylates 'Lys-310' in RELA to inhibit NF-kappa-B transcriptional activity, and deacetylates NR1D2 to counteract KAT5-mediated relief of NR1D2 transcriptional repression. By similarity, HDAC1 is part of an RCOR/GFI/KDM1A/HDAC complex that suppresses genes involved in multilineage blood cell development via histone deacetylase recruitment. It is also involved in CIART-mediated repression of the CLOCK-BMAL1 heterodimer and required for transcriptional repression of circadian target genes (e.g., PER1) by large PER or CRY1 complexes through histone deacetylation. Beyond protein deacetylase activity, HDAC1 exhibits protein-lysine deacylase activity, functioning as a decrotonylase and delactylase to mediate histone decrotonylation ((2E)-butenoyl) and delactylation (lactoyl), respectively.

Free

Q13547

Predicted band size: 55 kDa; Observed band size: 65 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102094

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2000; IHC-P: 1:50-1:800; ICC: 1:50

• 
  Western blot analysis of extracts from HEK293(lane2(20μg) , Hela(lane 3(20μg) and Jurkat(lane 4(20ug) using HDAC1 Antibody (HY-P80149) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling HDAC1with HDAC1 Antibody (HY-P80149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC1 Antibody (HY-P80149) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling HDAC1 with HDAC1 Antibody (HY-P80149) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC1Antibody (HY-P80149)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80149, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HDAC1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80149, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC1.AA range:420-460.