GRB2 Antibody (YA747)

GRB2 Antibody (YA747) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to GRB2.

Grb2, an adapter protein, plays a pivotal role in establishing a crucial connection between cell surface growth factor receptors and the Ras signaling pathway. Despite its lack of direct binding to phosphorylated epidermal growth factor receptor (EGFR), Grb2 exerts an inhibitory effect on EGF-induced transactivation of a RAS-responsive element. Notably, Grb2 functions as a dominant negative protein relative to GRB2, suppressing proliferative signals and potentially instigating active programmed cell death.

Free

P62993

Predicted band size: 25 kDa; Observed band size: 25 kDa

Protein G affinity purified.

Endosome, Nucleus, Golgi apparatus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102835

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat

WB: 1:500-1:1,000 ;ICC: 1:50-1:200 ;IHC-P: 1:50-1:200

• 
  Immunocytochemistry analysis of HepG2 cells labeling GRB2 with GRB2 Antibody (HY-P80147) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GRB2 Antibody (HY-P80147) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of MCF-7 cells labeling GRB2 with GRB2 Antibody (HY-P80147) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with GRB2 Antibody (HY-P80147)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat testis tissue using GRB2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80147, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat testis tissue using GRB2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80147, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Synthetic peptide corresponding to Human GRB2.AA range:21-217.