Glutathione Peroxidase 1 Antibody (YA749)

Glutathione Peroxidase 1 Antibody (YA749) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Glutathione Peroxidase 1.

Glutathione Peroxidase 1 (GPX1) is a glutathione-dependent enzyme that catalyzes the reduction of hydroperoxides, thereby regulating cellular redox homeostasis. It can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide, and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets, GPX1 also catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway. by similar

Free

P07203

Predicted band size: 22 kDa;Observed band size: 22 kDa

Protein G affinity purified.

Cytosol. Mitochondrion.

Non-conjugated

Unmodified

AB_3102318

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg), HepG2(lane 3(20μg) and HT-29(lane 4(20μg) using GPX1(HY-P80146) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, FC

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Synthetic peptide corresponding to Human Glutathione Peroxidase 1.AA range:20-203.