Glucocorticoid Receptor alpha Antibody (YA754)

Glucocorticoid Receptor alpha Antibody is a non-conjugated and Mouse origined monoclonal antibody about 86 kDa, targeting to Glucocorticoid Receptor alpha. It can be used for WB,IHC-P,FC,ICC assays with tag free, in the background of Human, Mouse.

The Glucocorticoid Receptor (GR) is a receptor for glucocorticoids (GC) with a dual mode of action: functioning as a transcription factor that binds to glucocorticoid response elements (GRE) in both nuclear and mitochondrial DNA, and as a modulator of other transcription factors. It regulates inflammatory responses, cellular proliferation, and differentiation, and is involved in chromatin remodeling. GR also promotes rapid mRNA degradation by binding to target mRNA 5' UTRs and interacting with PNRC2 in a ligand-dependent manner, recruiting UPF1 and DCP1A to induce RNA decay. Additionally, GR acts as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and plays a critical role in hepatic GR-mediated body growth regulation (by similar). GR exhibits both transcriptional activation and repression activities, mediates glucocorticoid-induced apoptosis, and ensures accurate chromosome segregation during mitosis. It may function as a tumor suppressor and negatively regulate adipogenesis by controlling lipolytic and antilipogenic gene expression (by similar). Different isoforms (e.g., Alpha) display varying functions, including transcriptional activity, hormone-binding capacity, and roles in glucose metabolism regulation.

Free

P04150

Predicted band size: 86 kDa； Observed band size: 100 kDa

Protein G affinity purified.

Cytoplasm. Nucleus.

Non-conjugated

Unmodified

AB_3102092

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;ICC: 1:50

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg) using Glucocorticoid Receptor alpha(HY-P80143) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, FC, ICC/IF

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding toHuman Glucocorticoid Receptor alpha.AA range:full length.