Glucocorticoid Receptor alpha Antibody (YA754)

Cat. No.: HY-P80143: Data Sheet COA
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Glucocorticoid Receptor alpha Antibody (YA754) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucocorticoid Receptor alpha.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Glucocorticoid Receptor alpha Antibody (YA754) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Glucocorticoid Receptor alpha Antibody (YA754) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucocorticoid Receptor alpha.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 86 kDa；

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P04150

Gene ID

2908 [NCBI]

Immunogen

Synthetic peptide corresponding toHuman Glucocorticoid Receptor alpha.AA range:full length.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50

Sensitivity	Endogenous	Purity	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB FC

Western blot analysis of extracts from HEK293(lane 2(20μg) using Glucocorticoid Receptor alpha(HY-P80143) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 A549 cells labeling Glucocorticoid Receptor alpha Antibody(HY-P80143, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

RRID

AB_3102092

Database

Entrez Gene: 2908 Human ; 14815 Mouse ;

SwissProt: P04150 Human ; P06537 Mouse ;

OMIM: 615962 Human

Research Field

Epigenetics and Nuclear Signaling

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Glucocorticoid Receptor alpha Antibody (YA754) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Glucocorticoid Receptor alpha Antibody (YA754)

Glucocorticoid Receptor alpha Antibody (YA754) Featured Recommendations:

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Glucocorticoid Receptor alpha Antibody (YA754) Related Classifications

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