GAPDH Antibody (YA848)

GAPDH Antibody (YA848) is a non-conjugated and Mouse origined IgM monoclonal antibody. It can be used as a loading control antibody.

GAPDH protein showcases a dual functionality with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, playing integral roles in glycolysis and nuclear functions, respectively. In glycolysis, it serves as a key enzyme catalyzing the initial step by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Beyond its metabolic role, GAPDH modulates the organization and assembly of the cytoskeleton and facilitates CHP1-dependent microtubule and membrane associations by stimulating the binding of CHP1 to microtubules (By similarity). It is also a component of the GAIT complex, which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. In innate immunity, GAPDH contributes to TNF-induced NF-kappa-B activation and type I interferon production by interacting with TRAF2 and TRAF3, respectively (By similarity). Furthermore, its involvement in nuclear events, including transcription, RNA transport, DNA replication, and apoptosis, is likely mediated by its nitrosylase activity, leading to cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and PRKDC (By similarity). The multifaceted functions of GAPDH underscore its versatile and critical roles in cellular processes.

Free

P04406

Predicted band size: 36 kDa; Observed band size: 36 kDa

Non-conjugated

Unmodified

AB_3102771

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Monkey, Rat, Mouse, Saccharomyces cerevisiae, Pichia pastoris, Hamster

WB: 1:5000-1:10000

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg), Hela(lane 3(20μg), K562(lane 4(20μg) ) ,RAW264.7(lane 5(20μg) ) using Hsp90 beta (HY-P80187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80187, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), C6 (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using GAPDH Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgM

Endogenous

Synthetic peptide corresponding to Human GAPDH.The exact sequence is proprietary to MCE.