FOXO4 Antibody (YA426)

FOXO4 Antibody (YA426) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXO4.

FOXO4 is a transcription factor involved in regulating the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Additionally, it plays a role in the negative regulation of the cell cycle. In embryonic stem cells (ESCs), FOXO4 enhances proteasome activity by activating the expression of PSMD11, leading to increased assembly of the 26S proteasome and consequently higher proteasome activity.

Free

P98177

Predicted band size: 54 kDa;Observed band size: 74 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102314

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Ramos(lane 2(10μg) and Ramos(lane 3(5μg), Jurkat(lane 4(5μg) ) and Jurkat(lane 5(10μg) ) using FOXO4(HY-P80134) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80134, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P, FC, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human FOXO4.AA range:456-505.