FOXO1A Antibody (YA429)

FOXO1A Antibody (YA429) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXO1A.

FoxO1 is a transcription factor that serves as the primary target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. It binds to the insulin response element (IRE) and Daf-16 family binding element (DBE), with its activity suppressed by insulin. FoxO1 is a key regulator of redox balance and osteoblast numbers, controlling bone mass (By similarity). It orchestrates the endocrine function of the skeleton in glucose metabolism regulation (By similarity). Under conditions of low lipid availability, FoxO1 translocates to the nucleus and promotes SOX9 expression, driving chondrogenic commitment while suppressing fatty acid oxidation (By similarity). FoxO1 synergizes with ATF4 to inhibit osteocalcin (BGLAP) activity, elevating blood glucose levels and triggering glucose intolerance and insulin resistance (By similarity). It also suppresses the transcriptional activity of RUNX2 (By similarity). In pancreatic beta cells, FoxO1 inhibits glucose sensing by repressing PDX1 expression (By similarity). In hepatocytes, FoxO1 promotes gluconeogenesis by co-activating genes such as IGFBP1, G6PC1, and PCK1 with PPARGC1A and CEBPA (By similarity). Additionally, it directly enhances PPARGC1A and G6PC1 expression to drive gluconeogenesis. FoxO1 is a critical regulator of cell death downstream of CDK1, PKB/AKT1, and STK4/MST1 (By similarity). It induces neural cell death and mediates insulin action in adipose tissue (By similarity). During preadipocyte differentiation, FoxO1 regulates adipogenic genes like PPARG and modulates adipocyte size and adipose-specific gene expression in response to excessive calorie intake (By similarity). It also governs GADD45A transcriptional activity and repairs nitric oxide-damaged DNA in beta cells (By similarity). In cardiomyocytes, FoxO1 mediates MLIP's role in hypertrophy and cardiac remodeling (By similarity). In cardiac smooth muscle cells, it promotes apoptosis by activating pro-apoptotic genes (By similarity). In endothelial cells (ECs), FoxO1 regulates viability and apoptosis via CCL2 and BCL2L11 transcription, which are involved in EC chemotaxis and apoptosis (By similarity).

Free

Q12778

Predicted band size: 70 kDa;Observed band size: 100 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102088

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:2000; ICC: 1:50-1:200; IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of A549 cells labeling FOXO1A with FOXO1A antibody (HY-P80132) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXO1A antibody (HY-P80132) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HeLa cells labeling FOXO1A with FOXO1A antibody (HY-P80132) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXO1A antibody (HY-P80132) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FOXO1A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80132, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FOXO1A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80132, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human FOXO1A.AA range:301-350.