FOXO1/FOXO1A Antibody

FOXO1/FOXO1A Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to FOXO1/FOXO1A.

The related gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of The related gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of The related gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Q12778

Predicted band size: 70 kDa; Observed band size: 78 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103190

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat, Pig

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; ChIP: 1:20; ELISA: 1:10000

• 
  Immunohistochemical analysis of paraffin-embedded rat brain tissue using FOXO1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat brain tissue using FOXO1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF, ELISA, ChIP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

The antiserum was produced against synthesized peptide derived from human FKHR.