FMRP Antibody (YA850)

FMRP Antibody (YA850) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FMRP.

FMR1 is a multifunctional polyribosome-associated RNA-binding protein that plays a central role in neuronal development and synaptic plasticity by regulating alternative mRNA splicing, stability, dendritic transport, and local protein synthesis. It mediates liquid-liquid phase separation to assemble mRNAs into cytoplasmic ribonucleoprotein granules, concentrating mRNAs with regulatory factors. FMR1 stabilizes DLG4/PSD-95 and MBP mRNAs in hippocampal neurons and glial cells, respectively, with enhanced stabilization upon mGluR stimulation (by similar). It selectively delivers dendritic mRNAs to synapses (by similar) and forms translation-repressive granules via phosphorylation and CAPRIN1 interaction. FMR1 acts as a translational repressor by stalling ribosomes (by similar) and participates in miRNA-mediated repression, while also activating translation of synaptic mRNAs (by similar). It binds numerous dendritic mRNAs, recognizing consensus sequences (e.g., 5'-ACU[GU]-3') and G-quadruplex structures. FMR1 facilitates nuclear export of m6A-modified mRNAs via XPO1/CRM1 and modulates ion channel activity (e.g., KCNT1, CACNA1B) and microtubule dynamics (by similar). It may influence DNA damage response (DDR) and positively regulates influenza A virus (IAV) replication by assisting viral ribonucleoprotein (vRNP) assembly and export.

Free

Q06787

Predicted band size: 71 kDa; Observed band size: 71-75 kDa

Protein A affinity purified.

Cell junction, Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Membrane, Nucleus, Postsynaptic cell membrane, Synapse, Synaptosome.

Non-conjugated

Unmodified

AB_3102777

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:1,000

• 
  Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using FMRP(HY-P80970 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling FMRP with FMRP antibody (HY-P80970) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FMRP antibody (HY-P80970) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling FMRP with FMRP antibody (HY-P80970) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FMRP antibody (HY-P80970) at 1/250 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FMRP Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80970, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FMRP Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80970, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice

IgG

Endogenous

Synthetic peptide within human FMRP aa 20-60.