FGFR2 Antibody (YA439)

FGFR2 Antibody (YA439) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGFR2.

FGFR2 is a tyrosine-protein kinase that functions as a cell-surface receptor for fibroblast growth factors (FGFs) and is essential for regulating cell proliferation, differentiation, migration, apoptosis, and embryonic development. It is critically involved in embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis, and skin development. FGFR2 phosphorylates substrates like PLCG1, FRS2, and PAK4 to activate downstream signaling cascades including the RAS-MAPK and AKT1 pathways, while its signaling is negatively regulated by ubiquitination, internalization, and degradation. Constitutive activation mutations or impaired maturation cause aberrant signaling, and overexpression promotes STAT1 activation. by similar: The protein stimulates proliferation in keratinocytes and immature osteoblasts but induces apoptosis in differentiated osteoblasts.

Free

P21802

Predicted band size: 92 kDa;Observed band size: 145 kDa

Protein A affinity purified.

Cell membrane, Golgi apparatus, Cytoplasmic vesicle.

Non-conjugated

Unmodified

AB_3102482

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000-1:2,000 ;IP: 1:10-1:50

• 
  Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using FGFR2 (HY-P80397) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80397, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human FGFR2.AA range:30-70.