FGFR2 Antibody (YA764)

FGFR2 Antibody (YA764) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FGFR2.

FGFR2 is a tyrosine-protein kinase that functions as a cell-surface receptor for fibroblast growth factors (FGFs) and is essential for regulating cell proliferation, differentiation, migration, apoptosis, and embryonic development. It is critically involved in embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis, and skin development. FGFR2 phosphorylates substrates like PLCG1, FRS2, and PAK4 to activate downstream signaling cascades including the RAS-MAPK and AKT1 pathways, while its signaling is negatively regulated by ubiquitination, internalization, and degradation. Constitutive activation mutations or impaired maturation cause aberrant signaling, and overexpression promotes STAT1 activation. by similar: The protein stimulates proliferation in keratinocytes and immature osteoblasts but induces apoptosis in differentiated osteoblasts.

Free

P21802

Predicted band size: 92 kDa；Observed band size: 140 kDa

Protein A affinity purified.

Cell membrane, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted.

Non-conjugated

Unmodified

AB_3102483

Cardiovascular

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:500 ;ICC: 1:100-1:200 ;IHC-P: 1:100-1:200

• 
  Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using FGFR2/CD332 Antibody (HY-P80398) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human FGFR2 / CD332.AA range:601-723.