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  5. EWSR1 Antibody (YA2270)

EWSR1 Antibody (YA2270)

Cat. No.: HY-P82525
COA
SDS
User Guide for Antibodies Technical Support

EWSR1 Antibody (YA2270) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2270), targeting EWSR1, with a predicted molecular weight of 68 kDa (observed band size: 85 kDa). EWSR1 Antibody (YA2270) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

EWSR1 Antibody (YA2270) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2270), targeting EWSR1, with a predicted molecular weight of 68 kDa (observed band size: 85 kDa). EWSR1 Antibody (YA2270) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.
Background

EWSR1 is a protein that binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (by similar). It may normally function as a transcriptional repressor. EWS-fusion proteins (EFPS) could play a role in tumorigenesis by disrupting gene expression through mimicking or interfering with the normal function of CTD-POLII within the transcription initiation complex, potentially leading to aberrant activation of fusion protein target genes.
Gene ID
SwissProt ID
Synonyms
EWSR1; EWS; RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein
Molecular Weight

Predicted band size: 68 kDa; Observed band size: 85 kDa
Purity

Affinity Chromatography
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Tags & Cell Markers
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

  • Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using EWSR1 (HY-P82525) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application

WB, IHC-P, ICC/IF
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human EWSR1
Database

Entrez Gene: 2130 Human ; 14030 Mouse ; 289752 Rat

SwissProt: Q01844 Human ; Q61545 Mouse ;

OMIM: 612219 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

EWSR1 Antibody (YA2270) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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EWSR1 Antibody (YA2270)
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