Estrogen Receptor alpha Antibody (YA3629)

Estrogen Receptor alpha Antibody (YA3629) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ESR1. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, rabbit.

ESR1 is a nuclear hormone receptor involved in regulating eukaryotic gene expression and affecting cellular proliferation and differentiation in target tissues. Its ligand-dependent nuclear transactivation involves direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors (e.g., AP-1/c-Jun, c-Fos, ATF-2, Sp1, and Sp3) to mediate ERE-independent signaling. Ligand binding induces a conformational change, enabling combinatorial association with multiprotein coactivator complexes through LXXLL motifs. ESR1 and NF-kappa-B mutually repress each other in a cell-type-specific manner, reducing NF-kappa-B DNA-binding activity and inhibiting NF-kappa-B-mediated transcription from the IL6 promoter while displacing RELA/p65 and coregulators. ESR1 is recruited to the NF-kappa-B response elements of CCL2 and IL8 promoters, displacing CREBBP. It coexists with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences and can synergize with NF-kappa-B to activate transcription via adjacent response element recruitment, a process requiring CREBBP. ESR1 also activates TFF1 transcriptional activity and mediates membrane-initiated estrogen signaling involving kinase cascades. It is essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). In the presence of circulating estradiol (17-beta-estradiol/E2), ESR1 maintains neuronal survival during ischemic reperfusion injury (By similarity) and is involved in NOS3 activation and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking functional domains modulate transcriptional activity via competitive ligand/DNA binding or heterodimerization with the full-length receptor (PubMed:10970861). ESR1 binds to ERE and inhibits isoform 1 (PubMed:10970861).

P03372

Predicted band size: 66 kDa; Observed band size: 66 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Rabbit

WB: 1/500-1/2000; IHC: 1/100-1/500; IF: 1/50-1/500; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human ESR1 (AA: 2-185) expressed in E. Coli.