Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

ERK1, a serine/threonine kinase, stands as a vital component in the MAP kinase signal transduction pathway, working in concert with MAPK1/ERK2 to orchestrate the MAPK/ERK cascade. This pathway, activated by stimuli such as KIT and KITLG/SCF, plays a pivotal role in diverse biological functions, including cell growth, adhesion, survival, and differentiation. ERK1, along with MAPK1/ERK2, influences transcription, translation, and cytoskeletal rearrangements, while also contributing to the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells through the phosphorylation of numerous transcription factors. With approximately 160 substrates identified for ERKs, their targets span various cellular compartments, participating in processes such as transcription regulation, translation, mitosis, and apoptosis. Additionally, the MAPK/ERK cascade is implicated in endosomal dynamics, lysosome processing, endosome cycling through the perinuclear recycling compartment (PNRC), and the fragmentation of the Golgi apparatus during mitosis. Noteworthy substrates include transcription factors, cytoskeletal elements, regulators of apoptosis, regulators of translation, as well as diverse signaling-related molecules, protein kinases, and phosphatases, thereby extending the cascade's specificity and amplifying its impact on cellular responses.

Free

P27361 / P28482

Predicted band size: 41/43 kDa; Observed band size: 41/43 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3102477

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:5,000-1:10,000； IF-Cell: 1:100; IHC-P: 1:1,000; IF-Tissue: 1:200; FC: 1:1,000; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IHC-P, ICC/IF, IP, FC, IF-Tissue

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human.AA range:166-215.