2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. EDD Antibody (YA2630)

EDD Antibody (YA2630)

Cat. No.: HY-P82885
User Guide for Antibodies Technical Support

EDD Antibody (YA2630) is a non-conjugated IgG antibody, targeting EDD, with a predicted molecular weight of 309 kDa (observed band size: 309 kDa). EDD Antibody (YA2630) can be used for WB experiment in human, mouse background.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
250 μL   Get quote  
Synthetic products have potential research and development risk.
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

EDD Antibody (YA2630) is a non-conjugated IgG antibody, targeting EDD, with a predicted molecular weight of 309 kDa (observed band size: 309 kDa). EDD Antibody (YA2630) can be used for WB experiment in human, mouse background.
Background

EDD is an E3 ubiquitin-protein ligase involved in various protein quality control pathways in the cytoplasm and nucleus. It primarily functions as a ubiquitin chain elongator, extending pre-ubiquitinated substrates. As part of the N-end rule pathway, EDD ubiquitinates proteins with specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation (by similar). EDD recognizes type-1 N-degrons containing positively charged amino acids (Arg, Lys, and His) (by similar). Together with UBR4, EDD participates in a cytoplasmic protein quality control pathway that prevents protein aggregation by catalyzing the assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, facilitating their recognition by p97/VCP and subsequent proteasomal degradation. EDD also collaborates with ITCH to catalyze 'Lys-48'-/'Lys-63'-branched ubiquitination of TXNIP, promoting its degradation. In the nucleus, EDD mediates ubiquitination and degradation of unpaired transcription factors (those not assembled into functional multiprotein complexes). Additionally, EDD regulates mRNA maturation and transcriptional regulation by polyubiquitinating and activating CDK9. It plays a role in DNA damage response by suppressing RNF168 to prevent excessive spreading of ubiquitinated chromatin at damaged chromosomes. EDD positively regulates the canonical Wnt signaling pathway by stabilizing CTNNB1 and degrading TLE3 via 'Lys-48'-linked ubiquitination. It is essential for extraembryonic development (by similar) and maintains skeletal tissue homeostasis by inhibiting hedgehog (HH) signaling (by similar).
Gene ID
SwissProt ID
Synonyms
DD5; EDD; EDD1; hHYD; HYD; Rat100; Ubiquitin protein ligase; UBR5
Molecular Weight

Predicted band size: 309 kDa; Observed band size: 309 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Cell Biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1/500-1/1000
Application

WB
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human EDD
Database

Entrez Gene: 51366 Human ; 70790 Mouse ;

SwissProt: O95071 Human ; Q80TP3 Mouse ;

OMIM: 608413 Human

Documentation
User Guide for Antibodies (1077 KB)

EDD Antibody (YA2630) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
EDD Antibody (YA2630)
Cat. No.:
HY-P82885
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.