E-Cadherin Antibody (YA470)

E-Cadherin Antibody (YA470) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E-Cadherin.

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact homophilically between connected cells, contributing to the sorting of heterogeneous cell types. CDH1 regulates epithelial cell-cell adhesion, mobility, and proliferation. By interacting with AMOTL2 (by similar), it organizes radial actin fiber structures and mediates cellular responses to contractile forces by anchoring these fibers to CDH1 junction complexes at the membrane. During early desmosome formation, CDH1 facilitates recruitment of DSG2 and DSP to desmosomal plaques. It acts as a potent invasion suppressor and serves as a ligand for integrin α-E/β-7. E-Cad/CTF2 promotes non-amyloidogenic processing of Aβ precursors and strongly inhibits APP C99/C83 production. Additionally (Microbial infection), CDH1 functions as a receptor for Listeria monocytogenes via binding of bacterial InlA to mediate internalization.

Free

P12830

Predicted band size: 97 kDa； Observed band size: 80~120 kDa

Protein A affinity purified.

Cell junction, Cell membrane, Endosome, Golgi apparatus.

Non-conjugated

Unmodified

AB_3102080

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from HT-29(lane 2(20μg), A431(lane 3(20μg) and HEK293(lane 4(20μg) using E-Cadherin (HY-P80113) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling E-Cadherin with E-Cadherin Antibody (HY-P80113)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with E-Cadherin Antibody (HY-P80113) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling E-Cadherin with E-Cadherin Antibody (HY-P80113) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with E-Cadherin Antibody (HY-P80113) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, FC, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human E-Cadherin.AA range:580-630.