DYKDDDDK Tag (FLAG) Antibody (YA780)

DYKDDDDK Tag (FLAG) Antibody is a non-conjugated and Mouse origined monoclonal antibody, targeting to DYKDDDDK Tag(FLAG). It can be used for WB,IP,IF assays with DYKDDDDK-tag, in the background of .

DYKDDDDK；FLAG Tag; flag；flag tag; Anti-Flag-tag

NA

Protein G affinity purified.

Non-conjugated

Unmodified

Tags & Cell Markers

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Species independent

WB: 1:5,000-1:10,000;IF: 1:100;IP: 2-5 µg/ml.

• 
  Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag (HY-P80111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, ICC/IF, IP

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to FLAG-tag.