Dnmt1 Antibody (YA476)

Dnmt1 Antibody (YA476) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dnmt1.

DNMT1 is a DNA methyltransferase that primarily methylates CpG residues, with a preference for hemimethylated DNA. During the S phase, it associates with DNA replication sites to maintain methylation patterns in newly synthesized strands, which is critical for epigenetic inheritance. In the G2 and M phases, DNMT1 binds to chromatin independently of replication to sustain DNA methylation. It plays a key role in preserving methylation patterns established during development and coordinates with histone methylation. DNMT1 mediates transcriptional repression by directly binding to HDAC2. In collaboration with DNMT3B and through the recruitment of CTCFL/BORIS, it participates in activating BAG1 gene expression by modulating dimethylation of histone H3 at H3K4 and H3K9 in the promoter region. In colorectal cancer (CRC) cells, DNMT1 likely forms a corepressor complex involved in KRAS-activated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes. Additionally, DNMT1 maintains a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) and binds to promoter regions of TSGs, leading to their silencing and promoting tumor growth.

Free

P26358

Predicted band size: 183 kDa; Observed band size: 183 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102824

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from HEK293(lane2(20μg) , HepG2(lane 3(20μg) and C6(lane 4(20ug) using Dnmt1 Antibody (HY-P80106) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HeLa cells labeling Dnmt1 with Dnmt1 Antibody (HY-P80106 )at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P80106 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling Dnmt1 with Dnmt1 Antibody (HY-P80106 ) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P80106 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Dnmt1.AA range:1506-1549.