DNA Ligase IV Antibody (YA2699)

DNA Ligase IV Antibody (YA2699) is a non-conjugated IgG antibody, targeting DNA Ligase IV, with a predicted molecular weight of 104 kDa (observed band size: 104 kDa). DNA Ligase IV Antibody (YA2699) can be used for WB experiment in human background.

DNA Ligase IV is a DNA ligase involved in DNA non-homologous end joining (NHEJ), playing a critical role in double-strand break (DSB) repair and V(D)J recombination. It catalyzes the NHEJ ligation step of DSB repair by resealing DNA breaks and can join single-strand breaks in double-stranded polydeoxynucleotides in an ATP-dependent reaction. LIG4 exhibits mechanistic flexibility: it can ligate nicks and compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotide (nt) microhomology and 1-nt gaps. LIG4 forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step, and XRCC4 enhances LIG4's joining activity. The binding of the LIG4-XRCC4 complex to DNA ends depends on the assembly of the DNA-dependent protein kinase complex DNA-PK at these ends. Additionally, LIG4 regulates the nuclear localization of XRCC4.

P49917

Predicted band size: 104 kDa; Observed band size: 104 kDa

Affinity Purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000

WB

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human DNA Ligase IV