Cytokeratin 5 Antibody (YA3509)

Cytokeratin 5 Antibody (YA3509) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 5.

CK5 is required for the formation of keratin intermediate filaments in the basal epidermis and maintains the skin barrier function in response to mechanical stress (by similarity). It potentially regulates the recruitment of Langerhans cells to the epidermis by modulating the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines, and CTNND1 localization in keratinocytes (by similarity).

P13647

Predicted band size: 62 kDa; Observed band size: 57 kDa

Affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

AB_3103013

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:5000; IHC-P: 1:50-1:1500; IF-Tissue: 1:200; ICC: 1:500; FC: 1:1000;

• 
  Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using Cytokeratin 5 (HY-P80984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984)at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within human Cytokeratin 5 aa 70-110.