Cyclin E2 Antibody (YA2594)

Cyclin E2 Antibody (YA2594) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2594), targeting Cyclin E2, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). Cyclin E2 Antibody (YA2594) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human background.

Cyclin E2 is a crucial protein involved in cell cycle regulation, primarily controlling the progression of the cell cycle during the late G1 and early S phases.

O96020

Predicted band size: 47 kDa; Observed band size: 47 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104942

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Hela (lane 2, 20 μg), Ramos (lane 3, 20 μg), and A431 (lane 4, 20 μg) using Cyclin E2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human Cyclin E2