Cyclin D1 Antibody (YA485)

Cyclin D1 Antibody (YA485) is a non-conjugated and Rabbit origined monoclonal antibody about 34 kDa, targeting to Cyclin D1. It can be used for WB,ICC/IF,IHC-P,IP assays with tag free, in the background of Human, Mouse, Rat.

Free

P24385

Predicted band size: 34 kDa; Observed band size: 35 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Membrane, Mitochondrion

Non-conjugated

Unmodified

AB_3102076

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2,000 ;ICC/IF: 1:50 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), NIH/3T3 (lane 4(20μg), NIH/3T3 (lane 5(40μg) using Cyclin D1 Antibody (HY-P80098) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80098, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Cyclin D1 with Cyclin D1 Antibody (HY-P80098) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cyclin D1 Antibody (HY-P80098) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
• 
  Immunocytochemistry analysis of Neuro-2a cells labeling Cyclin D1 with Cyclin D1 Antibody (HY-P80098) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cyclin D1 Antibody (HY-P80098)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Cyclin D1.The exact sequence is proprietary to MCE.