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  5. COX2 Antibody (YA791)

COX2 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 69 kDa, targeting to COX2. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Rat.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

Publications Citing Use of MCE COX2 Antibody (YA791)

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

COX2 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 69 kDa, targeting to COX2. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Rat.
Tag

Free
Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 69 kDa; Observed band size: 60 kDa
Purity

Protein G affinity purified.
Subcellular Location

Microsome membrane, endoplasmic reticulum membrane.
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Immunology
Product Categories

Primary Antibody; Mouse Monoclonal Antibody
Clonality

Monoclonal
Host

Mouse
Reactivity

Human, Rat
Dilution Ratio

WB: 1:500-1:1,000;IHC-P: 1:50-1:200;FC: 1:50-1:100

  • Western blot analysis of extracts from A549 (lane 2(20μg) , A549 (lane 3(40μg),using COX2 Antibody (HY-P80090). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application

WB, IHC-P, FC
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG1
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human COX2.The exact sequence is proprietary to MCE.
Database

Entrez Gene: 5743 Human ; 19225 Mouse ; 29527 Rat

SwissProt: P35354 Human ; Q05769 Mouse ; P35355 Rat

OMIM: 600262 Human

Documentation

COA (191 KB)

User Guide for Antibodies (1077 KB)

COX2 Antibody (YA791) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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COX2 Antibody (YA791)
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HY-P80090
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