Cleaved-PARP1 Antibody (YA500)

Cat. No.: HY-P80448: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Cleaved-PARP1 Antibody (YA500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-PARP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cleaved-PARP1 Antibody (YA500) Featured Recommendations:

Publications Citing Use of MCE Cleaved-PARP1 Antibody (YA500)

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Cleaved-PARP1 Antibody (YA500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-PARP1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 113 kDa;

Observed band size: 89 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P09874

Gene ID

142 [NCBI]

Immunogen

Synthetic peptide corresponding to Human PARP1.AA range:200-249.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500
FC FC: Flow Cytometry	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(20μg) using Cleaved PARP(HY-P80448) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

RRID

AB_3102525

Database

Entrez Gene: 142 Human

SwissProt: P09874 Human

OMIM: 173870 Human

Research Field

Epigenetics and Nuclear Signaling

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

Cleaved-PARP1 Antibody (YA500) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cleaved-PARP1 Antibody (YA500)

Cleaved-PARP1 Antibody (YA500) Featured Recommendations:

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Product Detail

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Background

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Cleaved-PARP1 Antibody (YA500) Related Classifications

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