Cleaved-PARP1 Antibody (YA500)

Cleaved-PARP1 Antibody (YA500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-PARP1.

Cleaved PARP is a poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. It catalyzes ADP-ribosylation of glutamate, aspartate, serine, histidine, or tyrosine residues, with serine ADP-ribosylation being the primary form in response to DNA damage. Specificity for different amino acids is conferred by interacting factors such as HPF1 and NMNAT1. In DNA break repair, Cleaved PARP recognizes and binds DNA breaks in chromatin, recruits HPF1, and promotes serine ADP-ribosylation of histones (e.g., H2BS6ADPr and H3S10ADPr), facilitating chromatin decompaction and repair factor recruitment. It also participates in double-strand break repair, transcription regulation, programmed cell death, membrane repair, adipogenesis, and innate immunity. During apoptosis, Cleaved PARP is cleaved by caspase-3 and caspase-7, translocates to the cytoplasm, and induces AIFM1-mediated apoptosis through auto-poly-ADP-ribosylation. This cleaved form irreversibly binds DNA breaks, interfering with DNA repair and promoting DNA damage-induced apoptosis.

Free

P09874

Predicted band size: 113 kDa; Observed band size: 89 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102525

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500; FC: 1:50-1:100; ICC: 1:50-1:200; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(20μg) using Cleaved PARP(HY-P80448) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, ICC/IF, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PARP1.AA range:200-249.