Chk1 Antibody (YA505)

Chk1 Antibody (YA505) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Chk1.

Chk1, a serine/threonine-protein kinase, plays a crucial role in orchestrating checkpoint-mediated cell cycle arrest and facilitating DNA repair in response to DNA damage or unreplicated DNA. Additionally, it exerts negative regulation on cell cycle progression during unperturbed cell cycles, employing various mechanisms to safeguard genome integrity. Chk1 recognizes the substrate consensus sequence [R-X-X-S/T] and phosphorylates key targets such as CDC25A, CDC25B, CDC25C, and RAD51. Notably, phosphorylation of CDC25A and CDC25C creates binding sites for 14-3-3 proteins, inhibiting their activity and impeding cell cycle progression. Chk1's intricate involvement extends to the phosphorylation of TP53, promoting its activation, leading to cell cycle arrest, and suppressing cellular proliferation. Furthermore, Chk1 phosphorylates histone H3.1, influencing epigenetic regulation of gene expression. Its multifaceted role encompasses the phosphorylation of RB1, SPRTN, TLK1, and FANCE, contributing to the regulation of transcription, chromatin assembly, and DNA repair processes. Chk1 emerges as a pivotal player in maintaining genomic stability and orchestrating cellular responses to diverse challenges.

Free

O14757

Predicted band size: 54 kDa; Observed band size: 54 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102294

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:2000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) and Hela(lane 3(40μg), using Chk1 (HY-P80082) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80082, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Chk1.AA range:431-476.