CEBP beta Antibody (YA1835)

CEBP beta Antibody (YA1835) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CEBP beta.

C/EBP Beta is a key transcription factor regulating genes involved in immune and inflammatory responses. It plays significant roles in adipogenesis, gluconeogenesis, liver regeneration, and hematopoiesis, with a consensus recognition site of 5'-T[TG]NNGNAA[TG]-3'. Its activity is modulated by protein interactions and post-translational modifications. During early embryogenesis, it shares redundant functions with CEBPA, promoting mitosis in hepatocytes and adipocytes while inhibiting T-cell proliferation by repressing MYC, thereby aiding T-helper 2 lineage differentiation. It binds regulatory regions of acute-phase and cytokine genes, influencing acute-phase reactions and inflammation. By similarity, it contributes to intracellular bacterial killing. In adipogenesis, it is rapidly expressed and, upon phosphorylation, induces CEBPA and PPARG to activate adipocyte phenotype-related genes. It is critical for ovarian follicle development (By similarity) and restricts osteoclastogenesis by repressing DSPP during odontoblast differentiation (By similarity). Essential for macrophage activation, it also drives immune responses like CD4(+) T-cell activation, granuloma formation, and endotoxin shock.

P17676

Predicted band size: 36 kDa; Observed band size: 36 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104025

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IF: 1/50-1/200;IP: 1/50;FC: 1/50-1/100

• 
  Immunocytochemistry analysis of NIH-3T3 cells labeling CEBPB with CEBPB antibody (HY-P82090) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEBPB antibody (HY-P82090) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HeLa cells labeling CEBPB with CEBPB antibody (HY-P82090) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CEBPB antibody (HY-P82090) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IP, FC

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human CEBP Beta