CDX2 Antibody (YA3498)

CDX2 Antibody (YA3498) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDX2.

CDX2 is a transcription factor that regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). It binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity), as well as the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR to activate VDR transcription (By similarity). Additionally, CDX2 binds to and activates transcription of LPH (By similarity), and also activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). It further binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). CDX2 plays a crucial role in a broad range of functions, from early differentiation to the maintenance of the intestinal epithelial lining in both the small and large intestine, and preferentially binds to methylated DNA.

Q99626

Predicted band size: 34 kDa;Observed band size: 40 kDa

Non-conjugated

Unmodified

AB_3103012

Stem Cells

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Immunocytochemistry analysis of HELA cells labeling CDX2 with CDX2 Antibody (HY-P80983) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDX2 Antibody (HY-P80983) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HELA cells labelingalpha CDX2 with CDX2 Antibody (HY-P80983) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDX2 Antibody (HY-P80983) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using CDX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using CDX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

Caco-2 cell lysate, LoVo cell lysate, AGS cell lysate, LOVO, AGS, human appendix tissue, Human stomach cancer tissue

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human CDX2