CD73 Antibody (YA528)

CD73 Antibody (YA528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD73.

CD73 is an enzyme that catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. It shows a preference for ribonucleotide monophosphates over their deoxyribose counterparts. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. by similar: The function and substrate specificity of CD73 have been validated in multiple studies.

Free

P21589

Predicted band size: 63 kDa;Observed band size: 75 kDa

Protein A affinity purified.

Cell membrane.

Non-conjugated

Unmodified

AB_3102110

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD73 (HY-P80387) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80387, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human CD73.AA range:491-536.